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Transmission Electron Microscope Imaging Service—JEOL JEM-1400Flash TEM

Transmission electron microscopy is an important tool for the observation and study of ultrastructure and is widely used in a variety of disciplines such as histology, cytology, virology, pathology, and materials science, for example, to observe the overall structure of cells, changes in cell membranes, organelles in cell walls, the distribution of materials into cells, autophagic vesicles produced by cells in response to external stimuli, and invasive structures invaded by external organisms. CD BioSciences' JEOL JEM-1400Flash TEM-based transmission electron microscopy imaging service enables customers to see the microscopic world.

Name of the Instrument/Platform

JEOL JEM-1400Flash TEM

MANUFACTURER:
JEOL

Introduction

Specifications of JEOL JEM-1400Flash TEM
1. Electron Source: LaB6 filament
2. Accelerating Voltage: 60 kv-120kv
3. Operation Modes: Bright Field/Dark Field/Diffraction
4. Magnification: Low Mag Mode 10× to 1,000×; Standard Mag Mode 200× to 1.2M×
5. TEM Resolution: 0.2 nm lattice; 0.38 nm point to point
6. Sample Holders: Jeol quick-change specimen holder with one specimen capacity; Fischione 2040 dual axis rotating holder
7. Tilt Angles: ±25° tilt with quick-change specimen holder; ±70° tilt with Fischione holder
8. Image Recording: AMT NanoSprint-43M-B Mid-Mount CMOS camera with a large 7915 × 5436 pixels format. This camera offers 5.5 μm square pixels at the phosphor and images a 43 × 30 mm physical FOV.

Important Notes

The JEOL JEM-1400 Flash TEM is equipped with:

1. Dual-Axis Fischione Model 2040 specimen holder, which allows for precise in situ specimen in-plane 360-degree rotation that is necessary for automated montaging and serial imaging. This specimen holder features an optimal tilt angle range in narrow gap (~ 5 mm) pole piece geometries, while maintaining microscope resolution. Thus, this holder can also be used for 3D tomography applications.
2. SerialEM and IMOD Software, which enables automated image acquisition, registration, and alignment of single- or dual axis tilt series. SerialEM also employs a versatile montaging system that can be used to acquire a large-scale image montage of the entire grid across the TEM sample. The IMOD software suite is a set of post-acquisition programs for processing the 3D tomography tilt series as well as serial section data into 3D reconstructions.

Summary

JEOL JEM 1400 Flash TEM system has (a) Dual-Axis Fischione Model 2040 specimen holder, which allows for precise in situ specimen in-plane 360 degree rotation that is necessary for automated montaging and serial imaging. This specimen holder features an optimal tilt angle range in narrow gap (~ 5 mm) pole piece geometries, while maintaining microscope resolution. Thus, this holder can also be used for 3D tomography applications; (b) SerialEM and IMOD Software, which enables automated image acquisition, registration, and alignment of single- or dual axis tilt series. SerialEM also employs a versatile montaging system that can be used to acquire a large-scale image montage of the entire grid across the TEM sample. The IMOD software suite is a set of post-acquisition programs for processing the 3D tomography tilt series as well as serial section data into 3D reconstructions.

CD BioSciences' transmission electron microscopy imaging laboratory has the professional equipment and experienced scientists. We are guided by the needs of our customers and are committed to promoting the progress of their research in biology and materials. Please feel free to contact us for any collaboration or questions.

For research use only, not intended for any clinical use.
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