Tissue Imaging Service—Zeiss Axiovert 200M Marianas Deconvolution System

Depth imaging in tissue is one of the greatest challenges facing optical microscopists. As the depth into the sample progressively increases, scattering and various optical aberrations gradually deteriorate, ultimately placing a practical limit on the possible imaging depths. CD BioSciences can solve your challenges with our advanced equipment and we offer Zeiss Axiovert 200M Marianas Deconvolution System-based tissue imaging services.

Name of the Instrument/Platform

Zeiss Axiovert 200M Marianas Deconvolution System

MANUFACTURER:

Zeiss

Introduction

System Specifications
• Microscope
1. Inverted Zeiss Axiovert 200M stand
2. DIC optics
3. Motorized stage
4. Environmental chamber for long-term imaging
• Light/excitation source
1. Lambda LS Xenon arc lamp (Sutter Instruments)
2. light output 340-700 nm
• Objectives
1. 5×/0.25 Air Fluar
2. 10×/0.3 Air EC Plan NeoFluar
3. 20×/0.50 Air Plan NeoFluar Ph 2 (1004-989)
4. 20×/0.50 Air Plan NeoFluar DIC (1004-072)
5. 40×/1.3 Oil Plan Neofluar
6. 63×/1.4 Oil Plan Apochromat DIC
• Fluorescence filter cubes
1. Blue (DAPI, Hoechst), Excitation filter 382-392 nm, emission filter 417-477 nm
2. Green (FITC, GFP), Excitation filter 465-499 nm, emission filter 516-556 nm
3. Red (TRITC, RFP), Excitation filter 532-554 nm, emission filter 573-613 nm
4. Far Red (Cy5, APC), Excitation filter 608-648 nm, emission filter 672-712 nm
• Detection
Photometrics' Coolsnap HQ2 cooled CCD camera (1392 × 1040 pixel imaging array)
• Acquisition software
1. SlideBook 6.0 (3i)
2. Experiment/image file format: SLD (e

CD BioSciences offers you a tissue imaging solution with unparalleled speed and excellent performance and flexibility, designed to provide the perfect research tool for the field of tumor immunology and digital pathology. If you are interested, please do not hesitate to contact us.

For research use only, not intended for any clinical use.

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