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Depth imaging in tissue is one of the greatest challenges facing optical microscopists. As the depth into the sample progressively increases, scattering and various optical aberrations gradually deteriorate, ultimately placing a practical limit on the possible imaging depths. CD BioSciences can solve your challenges with our advanced equipment and we offer TissueVision TissueCyte 1000-based tissue imaging services.
We also offer a fully developed, automated image analysis pipeline for use on whole brain volumetric images collected on the TissueCyte. The pipeline includes pixel classification using supervised machine learning, registration of fluorescent signals into the field standard average mouse brain atlas (Allen Institute for Brain Science Common Coordinate Framework, v3.0) and automated quantification of fluorescent signals of interest across every brain region. The fully automated nature of the TissueCyte serial two photon system coupled with the robust analysis pipeline is amenable to processing large numbers of experimental animal brains (mouse and rat). Automated analysis services are performed by our full-time computational scientist.
The state-of-the-art TissueCyte 1000 multi-photon microscopes with integrated precision vibratomes for automated sectioning and imaging of the entire volume of a mouse brain in approximately 10 hours. Typical resolutions are approximately 1 micron in the x-y dimensions and 50 to 100 microns in the z dimension, but with a combination of optical and physical sectioning, a z-resolution of 1 micron can be obtained. Brains from mice with a variety of fluorescent reporters can be imaged (e.g. transgenic animals expressing fluorescent proteins such as GFP, tdTomato or mice injected with fluorescent tracers). Perfusion-fixed mouse brains are delivered to the facility for imaging by our personnel.
CD BioSciences offers you a tissue imaging solution with unparalleled speed and excellent performance and flexibility, designed to provide the perfect research tool for the field of tumor immunology and digital pathology. If you are interested, please do not hesitate to contact us.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.