Tissue Imaging Service—Nikon 80I Epifluorescence Microscope

Depth imaging in tissue is one of the greatest challenges facing optical microscopists. As the depth into the sample progressively increases, scattering and various optical aberrations gradually deteriorate, ultimately placing a practical limit on the possible imaging depths. CD BioSciences can solve your challenges with our advanced equipment and we offer Nikon 80I Epifluorescence Microscope-based tissue imaging services.

Name of the Instrument/Platform

Nikon 80I Epifluorescence Microscope

MANUFACTURER:

Nikon

Introduction

System Specifications
• Microscope
Upright configuration
Manual stage
• Excitation source
X-Cite 120, Mercury vapor short arc
• Objectives
1. 4×/0.20 Air Plan Apo, WD 20 mm
2. 10×/0.45 Air Plan Apo, WD 4.0 mm, Cover glass 0.17 mm
3. 20×/0.75 Air Plan Apo, WD 1.0 mm, DIC, Cover glass 0.17 mm
4. 40×/0.95 Air Plan Apo, WD 0.14 mm, DIC Prism; Selectable cover glass 0.11-0.23 mm
5. 60×/1.4 Oil Plan Apo VC, WD 0.13 mm, Cover glass 0.17 mm
6. 100×/1.25 Oil Plan Apo, WD 0.17 mm, Cover glass 0.17 mm
• Fluorescence filter cubes
1. Blue (DAPI, Hoechst), Excitation filter 340-380 nm, dichroic mirror 400 nm, emission filter 435-485 nm
2. Green (FITC, GFP), Excitation filter 465-495 nm, dichroic mirror 505 nm, emission filter 515-555 nm
3. Red (TRITC, RFP), Excitation filter 540-580 nm, dichroic mirror 595 nm, emission filter 600-660 nm
4. Spectral, Excitation filters: 400-414 nm, 484-504 nm, 566-586 nm, transmission bands: 446-468 nm, 520-540 nm, 614-642 nm, emission filters: 446-468 nm, 520-540 nm

Technical Capabilities

1. Widefield fluorescence imaging
2. Brightfield image capture with color camera (i.e. H&E staining analysis)
3. Slide viewing

CD BioSciences offers you a tissue imaging solution with unparalleled speed and excellent performance and flexibility, designed to provide the perfect research tool for the field of tumor immunology and digital pathology. If you are interested, please do not hesitate to contact us.

For research use only, not intended for any clinical use.

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