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Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Olympus TIRF 4-Line STORM.
Microscope
Inverted research microscope Olympus IX-83 equipped with
1. motorized 8-position filter cassette
2. motorized revolving nosepiece
3. motorized ultrasonic xy-stage (IX3-SSU, Olympus)
4. hardware autofocus (IX3-ZDC2, 830 nm version, Olympus)
5. white LED (Thorlabs) for transmission light with electronic shutter
6. long working distance condenser IX2-LWUCD (working distance 27 mm, NA 0.55) with DIC prisms (60×, 100×)
7. DIC imaging via:
1) polarizer U-POT (added on top of condenser)
2) Dedicated DIC prisms for 60× (IX2-MDIC60) , and 100× (IX2-MDIC100) objectives
3) motorized DIC slider (below revolving noisepiece)
4) DIC analyzer IX3-DICTA (located in filter turret)
8. Microscope large incubator cellVivo from Pecon:
1) warm air controlled with TempController 2000-2: RT - 60℃, resolution: 0.01℃
2) Small stage top incubator for controlled CO2 as well as O2 concentration and 90 % humidity
3) CO2 and O2 is controlled via CO2
This fully-motorized microscope is equipped with a motorized 4-Line TIRF condenser, powerful lasers (405 nm, 532 nm, 488 nm, 561 nm, 640 nm, 730 nm) , and a highly sensitive EMCCD camera for single molecule experiments. A comprehensive software enables automated experiments and a complete control of the microscope hardware. An incubation chamber (temperature, CO2, and humidity control) provides live-cell imaging compatibility. By using an Image Splitter, simultaneous up to four color single molecule imaging is possible. In addition, the setup is equipped with a motorized stage and a hardware autofocus for fully automated experiments (multi-point acquisitions, overview/tile scans, multiwell screenings) which require long term focus stability. Three dimensional single molecule localization is realized by an astigmatic (cylindrical) lens which can be introduced into the emission light path.
CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.