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Optical Microscope Imaging Service—Olympus TIRF 4-Line SMT

Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Olympus TIRF 4-Line SMT.

Name of the Instrument/Platform

Olympus TIRF 4-Line SMT

Introduction

Microscope
Inverted research microscope Olympus IX-81 (manual) equipped with
1. motorized filter cassette
2. motorized revolving nosepiece
3. motorized z-drive with remote focus handle
4. hardware autofocus (z drift compensation module)
5. piezo z-stage (NanoScanZ, NZ100) from Prior
1) Range of motion: 100 µm
2) Repeatability: 1 nm
3) Accuracy: 0.5 % of travel
4) fully software-controlled
6. motorized xy-stage IM 120×80 from Märzhäuser
1) travel range: 120 × 80 mm
2) repeatability: < 1µm
3) resolution: 0.01 µm
7. 100 W halogen lamp for transmission light with electronic shutter
8. Mid-long working distance condenser IX2-LWUCD (working distance 27 mm, NA 0.55)
9. DIC imaging via:
1) polarizer U-POT (added on top of condenser)
2) Dedicated DIC prisms for 20× (IX2-MDIC20) , 60× (IX2-MDIC60) , and 100× (IX2-MDIC100) objectives
3) DIC slider U-DICTS (below revolving noisepiece)
4) DIC analyzer IX2-MDICT (located in filter turret)
10. Optional optovar lens: 1.6×
11. Microscope large inc

Summary

The combination of a fully-motorized microscope, a motorized 4-Line TIRF condenser connected to powerful lasers (405 nm, 488 nm, 561 nm, 640 nm) , and a highly sensitive and fast sCMOS camera provides a powerful setup for single molecule experiments. A comprehensive software enables automated experiments and a complete control of the microscope hardware. An incubation chamber (temperature, CO2, and humidity control) provides live-cell imaging compatibility. By using a Image Splitter, simultaneous multicolor imaging for single molecule tracking experiment is possible. In addition, the setup is equipped with a motorized stage and a hardware autofocus for fully automated experiments (multi-point acquisitions, overview/tile scans, multiwell screenings) which require long term focus stability.

CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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