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Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Olympus TIRF 3-Line.
Microscope
Inverted research microscope Olympus IX-71 equipped with
1. manual filter cassette
2. manual revolving nosepiece
3. manual z-drive
4. manual xy-stage
5. 100 W halogen lamp as transmission light source
6. Mid-long working distance condenser IX2-LWUCD (working distance 27 mm, NA 0.55)
7. Optional optovar lens: 1.6×
Objectives
1. LUMPLFLN 40×, NA 0.8, water immersion
2. UAPON 150× TIRF, NA 1.45, oil immersion
Olympus TIRF 3-Line
Fluorescence Illumination
1. 488 nm optically pumped semiconductor laser, max. power 200 mW (Sapphire LP, Coherent)
2. 561 nm diode-pumped solid state laser, max. power 200 mW (Cobolt Jive, Cobolt)
3.642 nm laser diode, max. power 140 mW (LuxX 642-140, Omicron)
4. All laser lines are coupled into laser fibers which are connected to a triple-line TIRF condenser from Olympus
Olympus TIRF 3-Line
DualView
Simultaneous acquisition of two emission channels via DualView DV2 from Photometrics
1. Each channel is projected onto one quadrant of the camera
2. C
This setup combines total internal reflection fluorescence microscopy (TIRFM) with powerful lasers and a highly sensitive electron multiplying CCD camera to enable super-resolution microscopy such as FPALM and STORM. By using a DualView, simultaneous multicolor imaging is easily accomplished. This allows for co-localization and co-locomotion studies of single molecules with high spatial resolution.
CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.