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Optical microscopy, allows us to see and understand the microscopic world beyond our perception. Today, optical microscopy is at the heart of many scientific and technical fields, including life sciences, biology, materials science, nanotechnology, industrial inspection, medicine, and many more. CD BioSciences, the imaging experts, can provide you with optical microscopy imaging services based on Nikon SIM (Ti2) (Inverted).
Techniques
1. 2D SIM
2. 3D SIM
3. 3D TIRF SIM
4. 3D SIM Stack
5. Live cell imaging
Instrument specifications
1. Inverted Nikon Ti2 microscope
2. Nikon PlanApo objectives 10×-20×, air
3. Nikon SR PlanApo IR 60× 1.27NA WI objective
4. Nikon SR Apo TIRF 100× 1.49NA Oil immersion objective
5. Four solid state lasers - 405 nm (violet, 67mW), 488 nm (blue, 120mW), 561 (yellow, 120mW), and 640 nm (red, 80mW)
6. Andor iXon897 EMCCD camera
7. Three & four colour grating blocks for 60× WI & 100× Oil objectives
8. Piezo stage
9. NIS Elements software controlling confocal acquisition and lasers
10. Tokai Hit stage top incubator
Housed on a state-of-the-art Ti2 microscope, our SIM platform can acquire images with a resolution down to 100nm. Various objectives are available for this system, including 100× oil immersion with motorized correction collar, and 60× WI objective, specialized for live cell imaging. It is a four colour platform (405 nm, 488 nm, 561 nm and 640 nm solid state lasers), where images are acquired on an Andor iXon 897 EMCCD camera.
In addition to this, the system is capable of imaging in either 2D SIM, 3D SIM, 3D stack SIM or TIRF SIM mode for fixed or live cells (Tokai Hit stage top incubation system). Stability of the field of view over the imaging period is enabled by the Piezo stage and Perfect Focus System (PFS).
CD BioSciences remains committed to providing you with a complete, state-of-the-art optical microscopy imaging service. If you are interested, please contact us directly.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.