Next-Generation Sequencing Service—PromethION P24

The main reason for using next-generation sequencing is the effective reduction in turnaround time with NGS compared to traditional methods. At the same time, next-generation sequencing makes it possible to analyze the transcriptome and genome of a species in a detailed and holistic way. CD BioSciences offers a growing range of services, from library construction to sequencing to complete experimental design and support. Our PromethION P24-based next-generation sequencing technology can help you advance genome engineering innovation.

Name of the Instrument/Platform

PromethION P24

Summary

A variety of preparation kits allow DNA, cDNA, or direct RNA to be sequenced. The input amount necessary for each type of sample varies according to the desired result. For the longest reads of genomic DNA, micrograms worth of clean starting material provide the highest quality of data, but not the highest amounts of reads. For amplicons including cDNA made from RNA, amplifying hundreds of nanograms should be simple, thus a lower input in the picograms should be reasonable.
During library preparation, adaptor consisting of motor protein and tether is ligated to the ends of RNA or DNA with tether furthest from the template. Adaptors can also be bought with barcodes for multiplexing. If tether interacts with a nanopore successfully, motor protein will shift the molecule into and through the protein nanopore. A voltage is set across the nanopore and the current will shift as base pairs move through the pore. Nanopores are arranged in an array such that multiple molecules can be sequenced

CD BioSciences, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.

For research use only, not intended for any clinical use.

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