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The main reason for using next-generation sequencing is the effective reduction in turnaround time with NGS compared to traditional methods. At the same time, next-generation sequencing makes it possible to analyze the transcriptome and genome of a species in a detailed and holistic way. CD BioSciences offers a growing range of services, from library construction to sequencing to complete experimental design and support. Our Illumina MiSeq™ System-based next-generation sequencing technology can help you advance genome engineering innovation.
Data Generation
1. Number of reads per run
1) Up to 25 million reads per run (eg, clusters passing filter)
2. Throughput per run
1) 25 GB data per 2 × 300 bp run (depending on the kit)
Instrumentation
1. Illumination
1) 12 light-emitting diodes at 520 nm, 650 nm
2. Instrument Control Computer
1) Base Unit: Intel Core i7-2710QE 2.10 GHz, 16 GB RAM Memory, 750 GB Hard Drive
3. Sequencing run time
2) ~ 5.5 hours for a 2 × 25 bp paired-read sequencing run using MiSeq Reagent Kit v2
3) ~ 24 hours for a 2 × 150 bp paired-read sequencing run using MiSeq Reagent Kit v2
4) ~ 39 hours for a 2 × 250 bp paired-read sequencing run using MiSeq Reagent Kit v2
5) ~ 21 hours for a 2 × 75 bp paired-read sequencing run using MiSeq Reagent Kit v3
6) ~ 56 hours for a 2 × 300 bp paired-read sequencing run using MiSeq Reagent Kit v3
7) ~ 19 hours for a 2 × 150 bp paired-read sequencing run using MiSeq Reagent Kit v2 Micro
8) ~ 17 hours for a 2 × 150 bp paired-read sequencing run using MiSeq Reagent Kit v2
The Illumina MiSeq™ System is a desktop next-generation sequencing (NGS) system from Illumina allowing fast and simple targeted or small genome sequencing. The main applications include targeted resequencing, metagenomic analysis and small whole-genome sequencing.
MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read length. The sequencing technology is based surface-based bridge amplification for clonal amplification and sequencing of each generated cluster using the Illumina "sequencing by synthesis" (SBS) method with reversible.
CD BioSciences, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.