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The main reason for using next-generation sequencing is the effective reduction in turnaround time with NGS compared to traditional methods. At the same time, next-generation sequencing makes it possible to analyze the transcriptome and genome of a species in a detailed and holistic way. CD BioSciences offers a growing range of services, from library construction to sequencing to complete experimental design and support. Our Applied Biosystems Quantstudio 5-based next-generation sequencing technology can help you advance genome engineering innovation.
qPCR is a method of measuring how much DNA in a sample contains a region (or regions) of interest. Like standard PCR, precisely-designed primers target specific regions that are (or may be) within the sample, and replicate that region over the course of 25-50 thermal cycles. Unlike standard PCR, the DNA is dyed with a fluorophore and the intensity of the fluorescence is measured at the end of each replication cycle. The cycle at which the fluorescence reaches measurable levels above background noise is referred to as the threshold cycle (CT). Control samples with known input quantities of DNA are run simultaneously, and their CT values are used to generate a standard curve so that the quantity of the experimental sample can be accurately calculated.
The QuantStudio 5 Real-Time PCR is a high-performance instrument equipped with a 384-well block. The system offers measurement of a broad range of genomic applications allowing use of probe-based assays and intercalating dyes.
CD BioSciences, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.