Next-Generation Sequencing Service—ABI Prism 3130×L Genetic Analyzer

The main reason for using next-generation sequencing is the effective reduction in turnaround time with NGS compared to traditional methods. At the same time, next-generation sequencing makes it possible to analyze the transcriptome and genome of a species in a detailed and holistic way. CD BioSciences offers a growing range of services, from library construction to sequencing to complete experimental design and support. Our ABI Prism 3130×L Genetic Analyzer-based next-generation sequencing technology can help you advance genome engineering innovation.

Name of the Instrument/Platform

ABI Prism 3130×L Genetic Analyzer

Applications

Plasmid and PCR sequencing of around 800 bp per reaction depending on template quality.
Microsatellite fragment analysis for fragments up to 2500 bases long.

Summary

The Applied Biosystems 3130 XL Genetic Analyzer is a state-of-the-art capillary-based instrument capable of moderate- to high-throughput DNA sequencing and fragment analysis (up to 192 samples/day). The 3130×L system is a fully automated, fluorescence-based capillary electrophoresis platform that simultaneously analyzes 16 samples. Our analyzer at the SGC is equipped with a capillary array capable of resolving a sequence about 800bp in length with high QV depending on template quality. The 3130×L is also equipped to perform microsatellite samples containing up to four different fluorescent labels. The system provides automated polymer loading, sample injection, separation and detection, and data analysis. The instrument can run unattended for up to 24 hours.

CD BioSciences, as a specialist sequencing company, has a large range of sequencers in our NGS Core, including Illumina MiSeq, NovaSeq, PacBio, and more. If you require this service, please contact us directly.

For research use only, not intended for any clinical use.

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