Neurobiology Imaging Service—Leica SP8X STED Microscope

Neuroscience is a multidisciplinary field involving the study of the structure and function of the nervous system. It aims to understand the development of cognitive and behavioral processes, as well as to understand various diseases (such as Alzheimer's or Parkinson's disease) and find treatments for them. Neuroscientists often need to use microscopy to study challenging and complex samples to better understand the nervous system. CD BioSciences offers a comprehensive range of Leica SP8× STED Microscope-based imaging solutions to enable you to overcome these challenges.

Name of the Instrument/Platform

Leica SP8× STED Microscope

MANUFACTURER:

Leica

Introduction

The Fluorescent Life Time (FLIM) imaging has the ability to measure the decay rate of your probes. As the fluorescence lifetime does not depend on concentration, absorption by the sample, sample thickness, photo-bleaching and/or excitation intensity it is more robust than intensity based methods.
The confocal is equipped with multiple excitation lasers including a continuum fiber laser that can deliver up to 8 simultaneous lines of any wavelengths between 470 and 670 nm. It has 4 detectors, two of which are high-sensitivity Hybrid detectors. It can be operated in STED, FLIM or standard confocal mode.

Feature Highlights

1. White light laser: continuous excitation tuning from 470 nm to 670 nm, Argon Ion Laser with 457, 477, 488 and 514 nm lines, 405 nm DPSS laser
2. 594 nm, 660 nm and 770 nm Depletion laser
3. 4 HyD and 3 PMT detectors
4. Transmitted light detector
5. Objective lenses:10× dry, 20× dry, 40× oil, 63× oil for confocal, 100× oil.
6. Tokai hit incubation chamber
7. Navigation for tilescans
8. Laser-based autofocus

Summary

The Leica 3× STED/FLIM system is a super resolution microscope based on the Leica SP8 confocal microscope. It has a guaranteed resolution of 100 nm though the system has been routinely achieving 50 nm. We recommend referencing the accepted dye list and sample prep notes by Leica for STED.

CD BioSciences' solutions can combine high resolution, deep imaging, and large section display to give you the flexibility to image different types of samples such as live cells, tissues, organoids, and model organisms. If this is of interest to you, please contact us directly.

For research use only, not intended for any clinical use.

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