Molecular Characterization Service—Thermo Fisher Scientific Versa 3D DualBeam

The characterization of molecules is an important part of the process, enabling the physical and chemical properties of molecules to be resolved. CD BioSciences has excellent analytical staff, years of analytical experience, and cutting-edge instrumentation. Using LC-MS, multiple HPLC, and other techniques, we can accurately quantify/characterize the DAR of coupled drugs, residual small molecules, endotoxins, and more.

Name of the Instrument/Platform

Thermo Fisher Scientific Versa 3D DualBeam

MANUFACTURER:

Thermo Fisher Scientific

Introduction

FEI Versa 3D Dualbeam Specifications
1. Electron Source: Schottky thermal field emitter (FEG).12 Month lifetime of the electron source
2. Accelerating Voltage: 200 V to 30 kV
3. Ion column: High-current Gallium liquid metal ion source. Source Lifetime 1300 hr/2,600uA
4. Ion probe current: 0.6 pA to 65 nA
5. Stage: 5 axes motorized. -15° to 90° tilt. Maximum sample size, 150 mm, 500g
6. 30kV SE Resolution: 1.2 nm
7. Ion beam resolution: 7 nm at 30kV
8. Angle between electron and ion beam columns: 52°
9. Specimen Exchange: Pump down time (high vacuum), approx.210 seconds
10. Operating Temperature: Room temperature; approx.21℃ ; cryo stage to -190℃ with cryo-shield
11. Gas Injection System (GIS): Platinum
12. SEM Imaging: Pixel scan size up to 6144 × 4096 pixels; 25 ns to 25 ms pixel dwell time

Applications

This versatile FIB-SEM instrument consists of a scanning electron microscope, applying an electron beam for imaging. In addition, the microscope is equipped with a second column producing a gallium ion beam. This allows to cut or ablate samples to ion beam mill material, microfabricate or produce cryo-lamellas for later observation or tomographic acquisition in a TEM.
The attached Quorum cold stage and preparation system allows imaging and FIB preparation of cryo-frozen material for optimal biological sample preservation without the need of chemical fixatives or dehydration.

Summary

Configuration
1. Large chamber DualBeam (FIB-SEM) instrument allowing Focused Ion Beam milling and Scanning Electron Microscopy. The SEM utilizes a Shottkey thermal field emitter (FEG) with accelerating voltages from 200V to 30kV.
2. 5 axis motorised stage
3. High throughput ion column optics with a high-current ion column applying an Galium liquid-metal ion source.
4. Platinum Gas Injection System
5. Low vacuum ESEM (environmental SEM) is possible.
6. Detectors include;
1) Everhart-Thornley SE detector (ETD).
2) Ion Conversion and Electron (ICE) detector for secondary ions (SI) and electrons (SE).
3) Retractable Directional Backscatter (DBS)*
7. Fitted with Quorum PP3010 cryo-SEM preparation system for imaging samples under cryo conditions, Stage cooled to -190℃ with cold trap. Preparation chamber vacuum down to 1×10-6mbar and has tools for freeze fracturing, sublimation and sputter coating.

CD BioSciences offers its customers Thermo Fisher Scientific Versa 3D DualBeam-based molecular characterization services that contribute to the process and drug development process of biomolecules. If you need our technical support, please contact us directly.

For research use only, not intended for any clinical use.

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