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Molecular and Cellular Analysis Services—Leica TCS SP8 LIGHTNING Confocal Microscope

The Molecular & Cellular Analysis Core is designed to assist users with molecular and cellular analysis of tissues, and gene expression studies including, but not limited to, primer and mutant design, amplification and quantification of RNA or DNA products, cloning, and other molecular biology techniques. To achieve this, we are equipped with a comprehensive range of instruments. Leica TCS SP8 LIGHTNING Confocal Microscope-based molecular and cellular analysis services can provide you with an unexpected experience.

Name of the Instrument/Platform

Leica TCS SP8 LIGHTNING Confocal Microscope

MANUFACTURER:
Leica

Feature Highlights

1. DM 6000 upright with EL 6000 fluorescence
2. Super Z Galvo stage, Type S
3. Objectives: 10× (HC PL FLUOTAR 10×/0.30), 20× (HC PL APO 20×/0.7 IMM CORR CS2), 25× (HCX IRAPO L 25×/0.95 W 0.17), 40× (HC PL APO 40×/1.30 Oil CS2), 63× (HC PL APO 63×/1.40 Oil CS2)
4. Filter sets:
5. Tandem resonant scanner 8 kHz
6. 2 PMT SP confocal detectors for imaging
7. 1 HyD SP GaAsP detector for gated imaging
8. Lasers: 405 nm DMOD Compact, Port UV/405, Blue 488 nm, Green 552 nm, Red 638 nm
9. LAS-X navigator software
10. LIGHTNING work station

Summary

The Leica TCS SP8 LIGHTNING confocal allows image information extraction with super resolution acquisition that exploits the sub-diffraction lateral resolution of confocal microscopy. It allows to capture cellular details and observe dynamics with a resolution down to 120 nm, which is close to STED super resolution microscope capabilities. The upright confocal is capable of simultaneous collection from up to 3 detection channels (two PMTs, one HyD) using multiple laser lines (diode 405, blue 488, green 552, red 638 nm). The confocal is configured with a conventional tandem scanner (8 kHz), a super Z-galvo stage type S, multiple objectives (10×, 20×, 25×, 40×, 63×), and stands on an anti-vibration table. The confocal is connected to a computer workstation equipped with Leica LAS-X software and a 30 inch monitor.

If you are interested in our Leica TCS SP8 LIGHTNING Confocal Microscope-based molecular and cellular analysis services, please contact us directly.

For research use only, not intended for any clinical use.
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