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Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Zeiss Elyra 7 (SIM2 upgraded) Lattice/SIM with TIRF-based confocal microscopy imaging services.
The Lattice SIM illumination allows a better signal to noise ratio, faster, gentler and can reach up to 255 fps during time lapse acquisition significantly faster than regular laser scanning confocal. It also allows a deeper imaging of samples up to around 70 µm thickness in comparison to 20 µm of sample thickness in classic SIM.
Lattice/SIM is a technology that can be used with standard fluorophores with the same protocol for confocal or regular wide-field fluorescence sample preparations, making it an easily accessible choice for super-resolution results.
Our Zeiss Elyra 7 also allows ApoTome imaging using 40× oil objective and lower. A grid based optical sectioning creates highly contrasted images with resolution comparable to SIM imaging and better than traditional laser scanning confocal. It allows to get lateral resolution ~170 nm and axial resolution ~450 nm. However, with the upgraded SIM2 module, apotome imaging can get lateral rsoltuion down to ~110 nm and axial resolution to
SIM is only available using the 63× oil and requires closely matching of refractive indices between the immersion oil and the mounting media of the specimen. A series of images are acquired with the illumination pattern in different orientations, giving spatial information that normally would be lost.
There are 15 composite images taken to generate a single 2D Classic SIM super- resolution image. The various illumination patterns require 3 rotations and 5 phase shifts for a total of 15 images in order to reconstruct one super-resolution image.
In Lattice SIM, The various illumination pattern orientations doesn't require rotations and a total of 13 or 15 images is required in order to reconstruct a single 2D Lattice SIM super-resolution image.
ApoTome Imaging is available using 10×, 20× and 40× objectives. There are 5 composite images to generate a single 2D ApoTome image. ApoTome mode is a faster mode that forgoes the illumination pattern rotations, instead using 5 phase shifts to quic
The Zeiss Elyra 7 is a wide-field based super-resolution system that uses a specialized 3D Lattice illumination pattern for 3D structured-illumination (SIM) images. It can reach up to ~120 nm lateral and ~300 nm axial resolution yields up to 2-fold improvement in voxel resolution in the X,Y, and Z dimensions. However, a new SIM2 module has recently been updated into the Elyra 7 that allows even higher resolution post-processing at ~60 nm lateral and ~200 nm axial resolution.
CD BioSciences' Zeiss Elyra 7 (SIM2 upgraded) Lattice/SIM with TIRF-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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