Microscope Imaging Service—Olympus Fluoview 1000 Spectral Confocal

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Name of the Instrument/Platform

Olympus Fluoview 1000 Spectral Confocal

Introduction

System details:
1.4 confocal PMT Detectors (photomultiplier tubes)
1) 2 detectors had with Spectral Detectors with 100 nm max bandwidth and 2nm resolution
2) 2 conventional filter detectors
2. A transmitted light detector
3. 6 laser lines for excitation
1) 405 nm 50mW diode
2) 440 nm 25mW diode
3) 488 nm 10mW Argon gas
4) 515 nm 10mW Argon gas
5) 559 nm 12mW pumped diode
6) 635 nm 20mW diode
4. 6 objectives standard in the turret
1) 10× and 20× air
2) 40× and 60× high refractive index oil
3) 30× and 60× silicon oil for imaging aqueous specimens
5. Fluorescence cubes for visualization (not used for imaging)
1) DAPI Like-Narrow UV EX360-370, DM400, EM420-460 U-MNIBA3(F)
2) CFP like -WIDE BV CUBE, EX400-440,DM455, EM475(U-MWBV2(F))
3) GFP/FITC like- NARROW IF BLUE CUBE, EX470-490, DM505,EM510-50 BAND (U-MNIBA3(F))
4) RFP/TRITC like-WIDE IF GREEN CUBE,EX520-550,DM565, EM580IF(U-MWIG3(F))
6. DIC-Differential Interference Contrast using transmitted light
7. DIC 10×, 20×, 40×, 60× prisms and

Summary

This system is an inverted Olympus IX81 confocal microscope equipped with 6 laser lines for excitation, spectral detectors and environmental control.

CD BioSciences offers Olympus Fluoview 1000 Spectral Confocal-based microscopy imaging solutions that are a powerful support for your research. If you are interested in our services, please feel free to contact us.

For research use only, not intended for any clinical use.

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