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Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Nikon Ti-HCS Microscope-based confocal microscopy imaging services.
Objectives
1. Nikon Plan Fluor 4× NA 0.13 (working distance 16.5 mm)
2. Nikon Plan Fluor 10× NA 0.3 phase contrast (working distance 16mm; corrected for 0.17 mm coverslips (= type #1.5))
3. Nikon Plan Apo 20× NA 0.75 (working distance 1.0 mm)
4. Nikon S Plan Fluor ELWD 20× NA 0.45 (working distance 8.2 - 6.9 mm; adjustable for glas and plastic bottom dishes with a thickness between 0 and 2 mm)
5. Nikon Plan Apo 40× NA 0.95 (working distance 0.25 - 0.17 mm)
6. Nikon S Plan Fluor ELWD 40× NA 0.60 (working distance 3.6 - 2.8 mm; adjustable for glas and plastic bottom dishes with a thickness between 0 and 2 mm)
Camera
1. Nikon DS-Qi2 high-sensitive CMOS monochrome camera with 2404 × 2404 pixel resolution (in total 4908×3264, the image is cropped to fit the FOV of the microscope) and a quantum efficiency of 80% at 500 nm.
2. With the DS-Qi2 camera at 2404 × 2404 pixel resolution, the following pixel sizes result: 4× 1.83 µm; 10× 0.73 µm; 20× 0.37 µm; 40× 0.18 µm.
Fluorescent light s
The Ti-HCS is a fully automated, inverted Nikon Ti2 microscope made for high content screening tasks. The microscope is encased by a environmental box from OkoLab for temperature-, CO2- and humidity control. By a software module names "JOBS" in the Nikon NIS-Elements acquisition software it is possible to screen in x/y, z and t everything from a LabTek dish to a multiwell plate. Images can be taken in brightfield and epifluorescence.
CD BioSciences' Nikon Ti-HCS Microscope-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.