Microscope Imaging Service—Nikon Ni-E Research Microscope

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Nikon Ni-E Research Microscop-based confocal microscopy imaging services.

Name of the Instrument/Platform

Nikon Ni-E Research Microscope

MANUFACTURER:

Nikon

Introduction

Objectives
All objectives are optimized for coverslips of the type #1.5 (meaning that they have a thickness of 0.17 mm). The usage of thicker (type #2) or thinner (type #1) coverslips may lead to suboptimal images. The 40× objective may be adjusted for coverslip thicknesses from 0.11 to 0.23 mm by a correction collar.
1. Nikon Plan Apo λ 2× NA 0.1 (working distance 8.5 mm)
2. Nikon Plan Apo λ 10× NA 0.45 (working distance 4 mm)
3. Nikon Plan Apo λ 20× NA 0.75 (working distance 1 mm)
4. Nikon Plan Apo λ 40× NA 0.95 (working distance 0.25 - 0.17 mm)
5. Nikon Plan Apo λ 60× NA 1.40 Oil (working distance 0.13 mm)
Filter Cubes
The Ni-E is equipped with the following filter cubes:
1. "DAPI": EX 390/18 DM 416 EM 460/60
2. "EGFP": EX 472/39 DM 495 EM 520/35
3. "TRITC": EX 543/22 DM 562 EM 593/40
4. "TexasRed": EX 562/40 DM 593 EM 624/40
5. "Cy5": EX 632/22 DM 660 EM 692/40
EX = excitation wavelength; DM = dichroic mirror (shorter wavelengths pass the mirror, longer wavelengths are reflec

Summary

The Nikon Ni-E is a fully motorized high-end upright (i.e. the objectives are facing downwards) research microscope mainly meant to image fixed samples that are either fluorescently labelled, histologically stained or untreated.
Fluorescent samples can be imaged in superior quality with a low noise, high sensitive Nikon DS-Qi2 black and white camera. Five filter cubes in the excitation/emission range of DAPI, FITC, TRITC, Texas Red and Cy5 are available. Histological sections may be imaged with a Nikon DS-Ri2 color camera that offers resolutions of up to 4908 × 3264 pixels. Untreated samples, even thin unlabelled cells, can be visualized by using Differential Interference Contrast (DIC). The Ni-E has a motorized stage that allows for automatic stitching: Areas on a slide larger than the field that a given objective can image, can be automatically captured as a series of frames, which are then seamlessly stitched together by the NIS-Elements acquisition software. So large images

CD BioSciences' Nikon Ni-E Research Microscop-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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