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Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with InVi-SPIM-based confocal microscopy imaging services.
The InVi-SPIM is a lightsheet microscope designed for live imaging and for smaller samples such as organoids. InVi-SPIM is the abbreviation for "Inverted View Selective Plane Illumination Microscope", i.e. the detection takes place from below, in contrast to the upright UltraMicroscope II. The sample holder is therefore freely accessible. Several samples can be placed next to each other and imaged sequentially. The required volume of medium is very small. The illumination is done by a 10× NA 0.3 objective (Nikon) , the detection by a 25× NA 1.1 objective (Nikon) on an Orca Flash 4.0 V3 C13440-20CU sCMOS camera with 2048×2048 pixels with a physical size of 6.5 × 6.5 µm. The QE reaches values above 80% in the medium visible wavelength range.
The InVi-SPIM is a lightsheet microscope for live imaging of smaller samples of up to 200 µm diameter. Signals can be detected in 3 channels (490, 561 and 640 nm excitation).
CD BioSciences' InVi-SPIM-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.