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Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Hitachi S-4700-based confocal microscopy imaging services.
1. Cathodoluminescence and Backscatter Electron Detectors
The S-4700 is equipped with a standard secondary electron detector (SE), a backscatter electron detector (BSD) and a Gatan MonoCL cathodoluminescence detector (CL). The BSD is sensitive to slight energy variations occurring when a high energy electron scatters off the nucleus of an atom. This sensitivity allows the BSD to map out variations in the densities of the sample. The CL measures photons released by the sample as it interacts with the electron beam and uses the information gathered from these photons to conduct elemental analysis.
2. Energy Dispersive X-ray Spectrographic Detector
In addition, the S-4700 includes a Bruker AXS Quantax 4010 Energy dispersive X-ray spectrometer (EDS). The EDS detector measures X-rays given off by sample as it is excited by the electron beam. Utilizing this data the Bruker software is capable of graphing the elemental make up of the sample, as well as creating a map of the image highlighting
The Hitachi S-4700 is a field emission scanning electron microscope (FE-SEM). More powerful than a standard SEM, the S-4700, under optimal conditions, can magnify images upwards of 500,000 times and resolve features to 2 nanometers. In addition, a cathodoluminescence detector, backscatter electron detector and energy dispersive x-ray spectrographic detector accompany the microscope.
CD BioSciences' Hitachi S-4700-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.