Email:
Tel: 
Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with ERS-VoX Spinning Disk Confocal Microscope + Widefield FURA Imaging-based confocal microscopy imaging services.
1. Components
Nikon automated Ti inverted microscope with
1) Nikon Plan Apo λ 100×NA 1.45 oil immersion objective (WD 0.13 mm)
2) Nikon Plan Apo λ 60×NA 1.40 oil immersion objective (WD 0.13 mm)
3) Nikon Plan Apo VC 60×NA 1.2 water immersion objective (WD 0.27 mm)
4) Nikon S Fluor 40×NA 1.3 oil immersion objective (WD 0.24 mm)
5) Nikon Plan Fluor 20×NA 0.75 multi immersion (water, glycerol, oil) objective (WD 0.35 mm)
Temperature-, CO2- and humidity control is provided by a TokaiHit on stage incubation system that can hold LabTek chamber slides and 35 mm dishes.
Hamamatsu C9100-02 EMCCD camera, 1000 × 1000 pixel, 8 µm pixel size
Lasers of 405, 488, 561 and 640 nm wavelength for excitation.
2. Detection
The filter sets in the ERS-VoX allow to detect fluorochrome emission in the following ranges (center wavelength [nm]/bandwidth [nm]) :
• "Green": 527/55
• "Blue/Red": dual pass filter 445/60 and 615/70
• "Cyan/Far Red": dual pass filter 485/60 and 705/90
• "Yellow": 587
The PerkinElmer ERS-VoX is a confocal microscope of the "spinning disk" (aka "nipkow disk") type. Here about 1,000 laser beamlets continuously sweep over the field of view, excite the fluorochromes in the sample, whose emission is recorded by a ultra sensitive EMCCD camera. Since each beamlet is quite dim, phototoxicity is relatively low. At the same time the quantum efficiency of a EMCCD camera is considerably higher than that of a photomultiplier used by a "normal" point scanning confocal microscope meaning that more photons can be detected. This allows for higher frame rates (up to 30 images per second) as opposed to the typical single frame per second achieved by point scanning devices.
A limitation comes from the fact that the pinholes required to gain confocality cannot be adjusted in size. Hence confocality is only obtained with a 100× oil immersion objective, limiting the field of view to about 66 × 66 µm. Also the penetration depth is smaller compared to a point scanning conf
CD BioSciences' ERS-VoX Spinning Disk Confocal Microscope + Widefield FURA Imaging-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
Please fill out the form below and we will get back to you as soon as possible with a quotation for the item you are interested in.
CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.