Immunological Assay Service—Leica BondRx Automated Stainer

An immunological assay is a biochemical test that measures the presence or concentration of large or small molecules in a solution through the use of antibodies or antigens. CD BioSciences has the extensive scientific knowledge and specialist instrumentation to provide powerful technical support for your analysis. We offer a Leica BondRx Automated Stainer-based immunoassay service.

Name of the Instrument/Platform

Leica BondRx Automated Stainer

MANUFACTURER:

Leica

Feature Highlights

1. Accelerate test programs through speed, efficiency, and consistent automation, reducing manual work.
2. Add signal amplification to almost any immunoassay. Improve sensitivity by 10 to 100-fold. 2 to 4 logs of Amplification dynamic range versus 1 log for chromogenic. And signal is more linear.
3. Excellent resolution with low background.
4. Reduce antibody consumption.
5. Unmodified primary antibodies
6. Use the best primary antibodies, regardless of species.
7. Multispectral imaging eliminates fluorophore crosstalk and interference from tissue autofluorescence, allowing precise measurement of each fluorescence signal within a tissue sample.
8. High-plexing for multiple biomarker detection strategies. The stain quality and reproducibility are better than the competition.

Technical Services

1. Consultation with your research projects.
2. Optimizations on the protocols.
3. Monoplex-multiplex staining up to 6 tissue biomarkers simultaneously plus a nuclear stain.
4. One batch of automated staining simultaneously up to 30 pieces of tissue slides.

Technical Capabilities

Functions
1. Fully automated IHC and ISH Research Staining.
2. In a single FFPE tissue section and frozen samples.
3. Monoplex and Multiplex Staining.
4. Opal kits measure up to 6 tissue biomarkers simultaneously plus a nuclear stain.
5. Retain spatial cellular context that is lost when using other methods.
6. Opal reagents utilize tyramide signal amplification (TSA) to increase antigen marker intensity by 10-100-fold compared to conventional indirect immunofluorescence methods.
7. Opal fluorescent detection substrates bind covalently near the epitope, allowing subsequent removal of antibodies to clear the tissue for detection of the next target. The signal remains intact after antibody removal.
8. A manual 4-color Opal staining protocol typically takes one day to complete and an automated 7-color can be run overnight, with all targets being imaged simultaneously.

CD BioSciences offers you a powerful collection of resources for your Leica BondRx Automated Stainer-based immunoassay service. If you are interested in our projects, please feel free to contact us.

For research use only, not intended for any clinical use.

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