Imaging Service—ZEISS Crossbeam 540

CD BioSciences is committed to providing one-stop services.Imaging service fully meets the needs of our clients to help them understand the tissue structure and elucidate the various physiological functions of living organisms. We can offer a wide range of biological services based on ZEISS Crossbeam 540.

Name of the Instrument/Platform

ZEISS Crossbeam 540

Introduction

A beam of ions, typically Ga+, is used to bombard a target material. It causes local sputtering of atoms at the target’s surface and hence removes material in a controlled manner.

Feature Highlights

Resolution: 0.9 nm @ 15kV (SEM) & 3 nm (FIB)
Accelerating Voltage: 0.1kV to 30 kV
FIB probe current: up to 40 nA
Attachments:
In-lens Secondary electron detector & Backscattered (EsB) electron detector
Secondary Electron and Secondary Ion imaging detector (SESI)
Annular Scanning Transmission Electron Microscopy (STEM) detector
Omniprobe manipulator
Pt & C gas injection systems
Energy Dispersive X-ray (EDS)
Samples for FIB operation should be restricted to dimensions of not larger than 10 mm x 10 mm x 5 mm (thickness). If not electrically conductive, they should be conductively coated to minimize sample drift during operation.

Applications

Cross-sectioning (cut flat section for looking into the material)
Rapid and site-specific TEM lamella preparation (cut a thin lamella at specific site for TEM)
Nanopatterning (i.e. deposition or milling of complex patterns/features)
Creating electronic circuit
3D tomography (cut and view the external and internal structures of a specimen in 3D space)

CD BioSciences offers you biological analysis services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

Related Services