Imaging and Analysis Services—Wide-Field NUANCE Microinjection Upright Microscope

CD BioSciences offers a unique range of high-end custom fluorescence microscopes, small animal imaging devices, and fluorescent probes to perform fluorescence-based measurements in a variety of spatial (nm to cm), and temporal (ns). Our laboratories offer consultancy services and support the application of novel spectroscopic methods and advanced microscopy techniques to achieve high spatial and temporal resolution nanotechnology from whole in vivo animal imaging to sub 70 nm imaging using super-resolution. In addition to using state-of-the-art specialist instrumentation, we train researchers in the use of advanced imaging techniques that are used to study macromolecules, and cell dynamics and characterize biological materials with nanometric precision.

Name of the Instrument/Platform

Wide-Field NUANCE Microinjection Upright Microscope

Introduction

With Nuance, it is no longer necessary to stain many serially sectioned slides to monitor the expression of multiple markers. Nuance can visualize multiple nuclear, cytoplasmic and membrane markers, all in the same tissue section, and all with intact morphology. Cell-by-cell expression of various markers can easily be appreciated, and captured quantitatively, providing information similar to that achievable with flow cytometry, but with the advantage that the physical location of markers, cells, and tissue architectural elements are maintained.
Nuance can spectrally characterize all the spectral components in a sample, and use this information to automatically separate and quantitate each signal into its own channel—for both fluorescence and brightfield immunohistochemistry.

Feature Highlights

1. Yokogawa CSU-X1 spinning disk confocal scanner unit
2. Software controlled motorized stage and piezo-focusing device for 3-D sectioning
3. Andor EMCCD camera.
4. Solamere Technologies laser bank, computer, Yokogawa scanner and computer with running software.
5. Laser bank: 4 solid state lasers with excitation lines at 405 (dark blue) 491 (blue-green), 561 yellow-green) and 640 nm (far red).
6. Fast PC computer with over a terabyte of data storage space.
7. Running software: MicroManager opensource microscopy management software. Ultra-fast image acquisition (up to 1000 fps in full-frame) with minimal photobleaching and phototoxicity.

Applications

1. Fluorescence imaging at all length scales: from single-molecule detection to in vivo small animal imaging.
The field of optical microscopy imaging is experiencing major technical advances which are allowing cell biologists and physicians to visualize a new, dynamic, sub-cellular world where genes and gene products interact in space and time, in health and disease with nanometer-accuracy.
2. Micro-and nano-scale imaging.
The facility currently provides: wide-field fluorescence imaging microscopy, stereo microscopy, iterative deconvolution and computationally derived optical sectioning, confocal one-photon and two-photon laser scanning confocal microscopy imaging, Fluorescence Correlation Spectroscopy (FCS), Fluorescence Resonance Energy Transfer (FRET), Fluorescence Lifetime Imaging (FLIM), time-correlated-single-photon-counting (TCSPC), near-infrared (NIR) imaging, and a super-resolution Stimulated Emission Depletion (STED) confocal microscope for probing biological and artif

Summary

The NUANCE multispectral imaging system is an enhancement to our wide-field microinjection upright to provides imaging of multiple molecular markers in tissue sections for both fluorescence and brightfield microscopy.

CD BioSciences offers Wide-Field NUANCE Microinjection Upright Microscope-based imaging and analysis services to researchers from all over the world. Our solutions will fully meet your experimental needs. We sincerely look forward to establishing a partnership with you. If you have any questions, please contact us directly.

For research use only, not intended for any clinical use.

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