Genomics Service—Droplet Digital Pcr (Ddpcr)–QX200

Rely on CD BioSciences' experienced genomics services to deliver high-quality, interpretable results using highly sensitive PCR, real-time PCR, NGS technologies, and advanced data analysis. All of your genomics research needs can be met with our highly flexible and customizable platform and our long experience in analyzing preclinical research samples.

Name of the Instrument/Platform

Droplet Digital Pcr (Ddpcr)–QX200

Introduction

Bio-Rad's QX200 Droplet Digital PCR (ddPCR) System consists of two instruments, the QX200 Droplet Generator and the QX200 Droplet Reader, plus their associated software and consumables. The QX200 Droplet Generator partitions samples (20 µL into 20,000 nanoliter-sized droplets) for PCR amplification. Following amplification using a thermal cycler, droplets from each sample are analyzed individually on the QX200 Droplet Reader, where PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. ddPCR measures absolute quantities by counting nucleic acid molecules encapsulated in discrete, volumetrically defined water-in-oil droplet partitions. Sample partitioning allows the sensitive, specific detection of single template molecules as well as precise quantification. It also mitigates the effects of target competition, making PCR amplification less sensitive to inhibition and greatly improving the discriminatory capacity of assays tha

Summary

The QX200 Droplet Digital PCR (ddPCR) system provides high-precision, absolute quantification of nucleic acid target sequences with wide-ranging applications for both research and clinical diagnostic applications.

CD BioSciences offers you Droplet Digital Pcr (Ddpcr)–QX200-based genomic services. If you are interested, please feel free to contact us for more information.

For research use only, not intended for any clinical use.

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