Electron Microscope Imaging Service—Titan Krios^™ G2 Transmission Electron Microscope

Electron microscopes can excite electrons to form images that magnify micron and nanostructures up to 10 million times, providing amazing levels of fine magnification and allowing researchers to observe even individual atoms. CD BioSciences can provide innovative solutions for electron microscopy and microanalysis. We offer Titan Krios^™ G2 Transmission Electron Microscope instruments that allow customers to combine high-resolution imaging with physical, elemental, chemical and electrical analysis to obtain usable data from the widest range of samples.

Name of the Instrument/Platform

Titan Krios^™ G2 Transmission Electron Microscope

Introduction

The Autoloader^™ sample loader allows for automatic loading of up to 12 samples. The flexibility of operating the Titan Krios G2 in the range of 80-300 kV allows for optimizing high tension to the requirements of the material under examination—ranging from vitrified suspensions to unstained cryo sections.
The Titan Krios is part of the Danish Cryo-EM national facility. Access is only granted upon selection of a project submitted via the Cryo-EM national facility portal.

Technical Capabilities

1. FEI Falcon^™ direct electron detector
The FEI Falcon Detector is based on direct electron detection using complementary metal oxide semiconductor (CMOS) technology. Due to the radiation damage and signal saturation, conventional charged-coupled devices (CCD) cannot be in direct contact with electrons. Therefore, CCD's rely on a scintillation layer to convert electrons to photons which can then be digitized. During the electron-to photon conversion process the spatial resolution of the information is reduced. FEI's Falcon Detector avoids the intermediate light conversion step required for conventional scintillated CCD detectors and as a result the spatial resolution of information is retained and digitized.
2. FEI Volta Phase Plate Ultimate Contrast Enhancement for 3D Cryo-TEM Imaging
FEI Volta Phase Plate increases contrast and retrieves more information from delicate biological samples. The FEI Volta Phase Plate is the ideal companion to demanding cutting-edge Cryo-TEM biological research.
3. EPU–Automated Single Particles Acquisition Software
EPU is a reliable, easy-to-use application that collects from tens of thousands up to millions of molecular views of a biological object in a quasi-automated fashion. Typically, it takes less than one hour to set up an EPU session that can run for days. It's possible to collect more than one million particles in a four-day session.

Summary

The Titan Krios^™ G2 transmission electron microscope (TEM) is tailored for use in protein and cellular imaging. Its cryo-based technology and stability permits a full range of semi-automated applications including:
1. Single particle analysis.
2. Dual-axis cellular tomography.
3. Cryo-electron microscopy.
4. 2D electron crystallography of frozen hydrated cell organelles and cells.
The Titan Krios G2 is a powerful and flexible high resolution electron microscope for 3D characterization of biological samples. The innovative enclosed platform design combines an excellent optical performance and thermal and mechanical stability.

CD BioSciences provides Titan Krios^™ G2 Transmission Electron Microscope-based electron microscopy imaging services to our clients. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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