DNA Shearing and Size Selection Services—Sage Science PippinHT

After DNA fragmentation or shearing, the library construction process for next-generation sequencing requires mainly platform-independent fragment selection. CD BioSciences offers Sage Science PippinHT-based DNA shearing and size selection services.

Name of the Instrument/Platform

Sage Science PippinHT

MANUFACTURER:

Sage Science

MODEL:

PippinHT

Feature Highlights

1. Generates higher-quality libraries for improved sequence informatics
2. Fragment sizes and ranges are reproducibly collected, providing consistent results, run-to-run
3. Flexible programming allows collection of multiple size ranges, or up to 24 collections of the same fragment range
4. The Pippin HT agarose cassettes available in the Core and the size ranges in which samples can be sized:
1) 75% from 6-10 kb, high -pass
2) 75% from 15-20 kb, high -pass
3) 75% from 30-40 kb, high -pass
4) 5% from 250 bp up to 1.5 kb
5) 2% from 100 bp up to 600 bp
6) 3% from 100 bp to 250 bp

Applications

• Size select DNA in ranges between 100bp up to 1.5kb.
• High-Pass protocols are available for collecting all larger DNA above a fragment size.

Summary

The Pippin systems allow for precise automated size selection of nucleic acid samples, sequencing libraries, and even protein samples. Pippin HT excels at high-throughput applications and also works with smaller sample amounts. Pippin HT uses disposable gel cassettes that are precast with agarose to perform DNA size selection and collect the desired DNA fragments based on user-input values.

If you are interested, please contact us directly for assistance.

For research use only, not intended for any clinical use.

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