DNA and Protein Quantification Services—Qubit 2.0 Fluorescence Reader

DNA and protein quantification is of great importance to life science research. CD BioSciences is an expert in the industry, with a wide range of advanced equipment and a wealth of experience and technology. We offer you Qubit 2.0 Fluorescence Reader-based DNA and protein quantification solutions.

Name of the Instrument/Platform

Qubit 2.0 Fluorescence Reader

Introduction

Specifications
1. The instruments has built-in programs for dsDNA (high sensitivity), dsDNA (broad range), RNA, ssDNA, and protein.
2. Sensitivities are:
1) dsDNA BR (broad range) 2-1000 ng
2) dsDNA HS (high sensitivity) 0.2-100 ng
3) ssDNA 1-200 ng
4) RNA HS (high sensitivity) 5-100 ng
5) protein 1-500 ng.
3. For more information on Qubit 2.0 reagents see the ThermoFisher web site.
4. Although the Nanodrops are advertised as sensitive to 2 ng/mL, they are not particularly accurate at such low concentrations. For samples below about 20 ng/µL we would recommend the Qubit reader. It is more sensitive and is not affected by UV absorbing materials such as free nucleotides that can result in an overestimation for DNA or RNA.

Applications

1. This popular instrument is a fluorescence reader for individual tubes that is used in conjunction with reagents kits to quantify dsDNA, ssDNA, RNA, and protein.
2. This reader is easy to use and much more sensitive that the Nanodrop and is the method of choice for quantifying Illumina libraries or low concentration samples.
3. Since it measures fluorescence (not aborbance) the Qubit does not detect UV absorbing materials such as free nucleotides, DNA contamination in RNA preps, or RNA contamination in DNA preps.
4. The Qubit is very simple to use and reagents and standards are supplied to users who are charged a modest cost per sample.

If you are interested in this service, please contact us directly.

For research use only, not intended for any clinical use.

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