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Cryo-electron microscopy (CryoEM) applies cryo-fixation technology to observe samples with a transmission electron microscope at low temperatures, to obtain the microscopic technology of the structure of biological macromolecules. After the piece is frozen and placed in the electron microscope, the electrons are scattered through the sample and the nearby ice layer. Use the detector and lens system to image and record the scattered signal, then process the signal, and finally use the three-dimensional reconstruction technology to obtain the three-dimensional structure of the sample. CryoEM imaging services are provided by CD BioSciences based on Thermo Fisher-FEI Titan Krios.
Performance Metrics
In order to validate performance, our instruments are routinely tested to insure high-resolution instrument performance. The FEI Titan Krios performance has been validated to 1.9 Angstrom structural resolution using Apoferritin as a well-studied target structure utilizing our Gatan K2 detector and Gatan Energy Filter combination. Customized microscope suites are providing stable environmental conditions to demonstrate 0.65 Angstrom point resolution. Further benchmarking with the Continued benchmarking and performance metrics are ongoing in order to determine optimum imaging and processing conditions.
The FEI Titan Krios is a 300keV ultra-high- performance, automated, three condenser transmission electron microscope is designed specifically for cryo electron microscopy and is capable of delivering 1.8 Angstrom atomic resolution structures. The system is equipped with two direct detector systems. The pre-filter Falcon 3 device's counting mode and drift correction produce high signal-to-noise and high-resolution images for tomography and single particle data. Additionally, the ultrahigh framerate and super-resolution modes of the energy filtered Gatan K2 camera system provide fast, high-resolution filtered imaging possibilities. The energy filter providing unmatched contrast. The system is equipped with a Volta phase plate that makes imaging of very small (less than 200kDa) proteins
CD BioSciences offers its customers the CryoEM imaging service based on Thermo Fisher-FEI Titan Krios to help analyze the 3D structure and microstructure of samples. If you are interested, please feel free to contact us.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.