Confocal Microscope Imaging Service—Nikon A1Si

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with Nikon A1Si-based confocal microscopy imaging services.

Name of the Instrument/Platform

Nikon A1Si

MANUFACTURER:

Nikon

Introduction

Techniques
1. Four colour confocal microscopy
2. Multipoint and live time-lapse imaging using Tokai Hit incubation chamber
3. Spectral detection and unmixing
4. Transmitted light DIC imaging
Instrument specifications
1. Inverted Nikon Ti E microscope
2. Objectives 4×-60×, air, water and oil immersion
3. Four 50mW solid state lasers - 405 nm (violet), 488 nm (blue), 561 nm (yellow), and 638 nm (red).
4. Four PMT detectors
5. 32-channel spectral detector
6. Total internal reflection fluorescence (TIRF) - two colour (488 nm and 561 nm)
7. 885 EMCCD camera for high-speed image capture
8. NIS Elements software
9. Perfect focus system (PFS) for time-lapse imaging
10. Tokai Hit incubator for time-lapse imaging

Summary

Nikon Ti-E inverted motorised microscope with Nikon A1Si spectral detector confocal system running NIS-C Elements software.
The system is capable of simultaneous or sequential image collection using four photomultiplier fluorescence detectors, a 32-channel spectral detector and a transmission detector. Long-term time-lapse imaging is possible using the Tokai Hit stage top incubation system.
Two colour (488 nm and 561 nm) total internal reflection fluorescence (TIRF) microscopy is integrated into the microscope and high-speed image capture is available using the Andor 885 EMCCD camera, all of which is controlled by the NIS Elements software.

CD BioSciences' Nikon A1Si-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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