Confocal Microscope Imaging Service—M38-SP8 DIVE Confocal (Inverted) with Multiphoton Lasers and Incubation

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with M38-SP8 DIVE Confocal (Inverted) with Multiphoton Lasers and Incubation-based confocal microscopy imaging services.

Name of the Instrument/Platform

M38-SP8 DIVE Confocal (Inverted) with Multiphoton Lasers and Incubation

Introduction

1. Widefield Illumination
1) LED
2) LED for brightfield
2. Lasers for Confocal Imaging
1) Laser Diode 405
2) Argon 458, 476, 488, 496, 514
3) DPSS 561
4) HeNe 633
3. Lasers for multiphoton imaging
1) Spectra Physics InSightX3
2) Tuneable range: 680-1300 nm
3) Fixed: 1045 nm
4. Detection range
1) Confocal detection 350 - 800 nm
2) 4 Tune Detector 380-800 nm
5. Objectives
1) 10×/0.3 HC PL Fluotar WD - 11 mm
2) 25/0.95W Fluotar VISIR WD - 2.4 mm
3) 40×/1.1W HC PL IR API WD - 0.65 mm
4) 63×/1.3 Glycerol HC PL APO CS2 WD - 0.3 mm
6. Additional Devices
1) XY Motorized Stage
2) Z - Galvo stage
3) Multiple Position Acquisition
4) High Dynamic Range acquisition (HDR)
5) Incubation with CO₂

Summary

The M38 system is an inverted point laser scanning confocal microscope. It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. SP8 DIVE features a highly sensitive HyD (Hybride detector) detectors with a high quantum efficiency and low noise as compared to standard PMTs. The system is equipped with a motorized xyz-stage and a hardware autofocus device. M38 is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.
In addition to the classical single photon excitation the M38 is equipped with a multiphoton laser and a spectral, non descanned (NDD) 4 Tune detector. The NDD detector contains 3 HyDs and 1 PMT. The emission light is separated by a combination of variable dichroics and bandpass filters over the whole visibl

CD BioSciences' M38-SP8 DIVE Confocal (Inverted) with Multiphoton Lasers and Incubation-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

Related Services