Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with M24-LSM780 Axio Imager (Upright)-based confocal microscopy imaging services.
1. Widefield Illumination
1) LED - 400 nm, 465 nm, 555 nm
2) Halogen Lamp 50W
2. Lasers for Confocal Imaging
1) Laser Diode 405 25mW
2) Argon 458, 488, 514 30mW
3) DPSS 561 15mW
4) HeNe 633 5mW
3. Filters
1) CH1: 371-735
2) CH2: 479-735
3) CH3 Quasar (GaAsP) : 416-690 in 8.3 nm steps
4) CHD: T-PMT (transmitted light)
4. Objectives
1) 10×/0.3 plan-apochromat
2) 20×/0.8 plan-apochromat
3) 25×/0.8 LD LCI plan-apochromat, multiimmersion DIC
4) 40×/1.3 EC plan-apochromat Oil DIC
5) 63×/1.2 plan-apochromat
6) 63×/1.4 plan-apochromat Oil DIC
7) Water dipping objectives for live-cell imaging40×/0.75W Achroplan
8) 63×/0.9W Achroplan
9) 100×/1.0W Achroplan
5. Additional Devices
1) XY Motorised Stage, Multiple Position Acquisition.
The M24 system is an upright point laser scanning confocal microscope (LSM780) . It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. Designed for live cell imaging, LSM780 features high sensitive array of 32 GaAsP (Gallium Arsenide) detectors with QE of 45% and up to 2× SNR.
The system is equipped with a motorized xy-stage, it´s fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.
CD BioSciences' M24-LSM780 Axio Imager (Upright)-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
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