Confocal Microscope Imaging Service—M21-LSM 710 2Pi Axio Observer (Inverted) with Incubation

Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. CD BioSciences, the imaging experts, can provide you with M21-LSM 710 2Pi Axio Observer (Inverted) with Incubation-based confocal microscopy imaging services.

Name of the Instrument/Platform

M21-LSM 710 2Pi Axio Observer (Inverted) with Incubation

Introduction

1. Widefield Illumination
1) X-Cite Lamp 120W
2) Halogen Lamp 50W
2. Lasers for Confocal Imaging
1) Laser Diode 405 25mW
2) Argon 458, 488, 514 30mW
3) DPSS 561 15mW
4) HeNe 633 5mW
5) Chameleon 2Pi - 705-980 nm
3. Filters
1) CH1: 371-740
2) CH₂: 479-750
3) CH3 Quasar:416-728 in 10 nm steps
4) CHD: T-PMT (transmitted light)
5) BiG detector: BP500-550 & BP570-630
NDD:
BiG2 detector: BP500-550 & BP600-650
4. Objectives
1) 10×/0.3 EC plan-neofluar
2) 20×/0.8 plan-apochromat
3) 25/0.8 LD LCI plan-apochromat, multiimmersion DIC
4) 40×/1.3 EC plan-neofluar Oil DIC
5) 63×/1.4 plan-apochromat Oil DIC
5. Additional Devices
Incubation chamber with CO₂ control, XY motorized stage, Multiple Position Acquisition

Summary

The M21 system is an inverted point laser scanning confocal microscope (LSM710) . It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. The system is also equipped with the Chameleon 2-Photon laser and the non-descaned detectors (NDD) as well as a motorized xy-stage.
The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.
This makes it suitable e.g. for a xyzt-experiments on up to 4 slides, using a special stage insert and the 2-Photon Laser is especially suitable for deep tissue imaging.

CD BioSciences' M21-LSM 710 2Pi Axio Observer (Inverted) with Incubation-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.

For research use only, not intended for any clinical use.

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