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Biopolymers are polymers produced by living organisms. Because they are polymers, biopolymers contain monomeric units that are covalently bonded to each other to form larger structures. CD BioSciences uses analytical separation techniques with advanced detectors to structurally characterize biopolymers.
The SEC-MALS 20 multi-angle light scattering detector is connected to the output of an HPLC system equipped with an SEC column. Signals generated from the sample passing through all detectors are analyzed by OmniSEC software consisting of 20 detectors arranged around a vertical flow cell to detect scattered light at a wide range of angles. The angular intensity and concentration information is combined to construct a plot, which is used in two ways. Firstly to extrapolate the data to determine the scattered light intensity at zero angles, from which the absolute molecular weight is calculated. Secondly to determine the initial slope of the plot which is related to the radius of gyration of the molecule (Rg). This system is equipped with a Viscotek VE3580 RI detector for the accurate determination of sample concentration.
The Viscotek SEC-MALS 20 is a multi-angle light scattering detector that can be used to measure the absolute molecular weight and oligomeric state of proteins, independent of retention time. It can also be used to analyze natural and synthetic polymers, including the measurement of molecular size over the widest possible range expressed as the radius of gyration, Rg.
CD BioSciences offers you Malvern Viscotek SEC-MALS 20 based biopolymer characterization services. If you are interested, please contact us directly.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.