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Biological Imaging Service—Zeiss PALM MicroBeam Laser Capture Microdissection Microscope

Biological imaging is an important research tool for understanding tissue structure and elucidating the various physiological functions of living organisms. It uses light or electron microscopy to obtain direct images of the microstructure of biological cells and tissues, and the resulting images are analyzed to understand the various physiological processes of biological cells. CD BioSciences' Bioimaging Centre has dozens of devices and, more importantly, our expertise and experience are invaluable to the industries and clients we serve. We can provide Zeiss PALM MicroBeam Laser Capture Microdissection Microscope-based bioimaging services.

Name of the Instrument/Platform

Zeiss PALM MicroBeam Laser Capture Microdissection Microscope

MANUFACTURER:
Zeiss

Introduction

Cameras:
• Color: Axiocam 503 color
• Fluorescent: Axiocam 503 mono
Operating Software:
• PALM Robo 4.8
• ZEN Blue 2.6 Pro
• AxioVision SE64 4.9.1
Fluorescent Filter Sets:
• Blue/DAPI
• Green/FITC
• Red/TRITC
• Far Red/Cy5
Objectives:
• 5× Fluar/0.25
• 10× Fluar/0.5, DIC II
• 20× LD PlanNeofluar/0.4, DIC II
• 40× LD PlanNeofluar/0.6, DIC II
• 63× LD PlanNeofluar/0.75, DIC II
• 100× PlanNeofluar/1.3, Oil, DIC III

Summary

The Zeiss PALM MicroBeam Laser Capture Microdissection (LCM) Microscope is a dual use system.
1. As an LCM, it uses a pulsed solid-state 355 nm (UV-A) laser for isolating specific cells for downstream analysis. It can isolate DNA, RNA, proteins, chromosomes, single cells, small organisms and live cells. Samples for LCM can be viewed in either brightfield or fluorescence mode. The LCM can isolate samples on slides or in culture dishes. Supporting substrates can be either standard glass microscope slides or special membrane slides (PEN or PET slides). PET membrane slides are ideal for fluorescence work. The LCM is compatible with both cryostat-sectioned or formalin fixed and paraffin embedded tissue. The Palm Robo software is used for LCM collection.
2. The system can also be utilized as an inverted microscope for both brightfield and fluorescent imaging. Advanced imaging techniques such as tile sets, Z stacks and time series are capable using the ZEN Blue software.

In recent years, with the development of optical imaging technology, especially the introduction of digital imaging and computer image analysis, bioimaging has become an indispensable method in cell biology research. CD BioSciences' bioimaging laboratory is committed to providing state-of-the-art technical support to our customers. If you are interested, please do not hesitate to contact us.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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