Biological Imaging Service—Zeiss LSM 880 NLO Two Photon

Biological imaging is an important research tool for understanding tissue structure and elucidating the various physiological functions of living organisms. It uses light or electron microscopy to obtain direct images of the microstructure of biological cells and tissues, and the resulting images are analyzed to understand the various physiological processes of biological cells. CD BioSciences' Bioimaging Centre has dozens of devices and, more importantly, our expertise and experience are invaluable to the industries and clients we serve. We can provide Zeiss LSM 880 NLO Two Photon-based bioimaging services.

Name of the Instrument/Platform

Zeiss LSM 880 NLO Two Photon

MANUFACTURER:

Zeiss

MODEL:

Zeiss LSM880 Two Photon

Introduction

1. Laser Lines
1) Argon: 458, 488, 514 nm
2) Diode: 405 nm.
3) DPSS: 561 nm
4) HeNe: 633 nm.
5) Mai Tai DeepSee Tunable Laser: 690-1040 nm.
2. Objectives
1) Plan Apo 10×/0.45 NA WD=2.0 mm
2) Plan Apo 20×/1.0 NA water WD=2.3 mm
3) Plan Apo 40×/1.0 NA Water WD=2.5 mm
4) Clarity Plan Neofluar 20×/1.0 NA WD=5.6 mm
3. Detectors
1) 2 PMT's
2) 1 GaAsP (2× sensitivity)
3) 1 Airyscan super resolution detector
4) 1 Transmitted light detector
5) 2 Channel GaAsP detector for 2 Photon

Feature Highlights

1. Airyscan to achieve 1.7× resolution (140 nm XY, 400 nm Z) and 4-8× sensitivity
2. FAST model for 4× speed up to 100fps
3. Mai Tai DeepSee 2 photon for deep tissue imaging
4. Axio Observer upright platform with motorized XYZ stage
5. Clarity 20× objective with 5.6 mm working distance
6. Zen Black software with the modules of Z stacks, timelapse, tiling, multi position, co localization, FRAP, super resolution, deconvoltuion, 3D processing.

Applications

1. Super resolution confocal deep imaging of fixed tissues or live samples
2. Fluorescence Correlation Spectroscopy (FCS)
3. Capturing fast events such as Ca²⁺ imaging
4. FRAP
5. FRET

Summary

The state of the art Zeiss LSM 880 NLO upright two photon confocal microscope is equipped with tunable Spectra Physics Mai Tai HP DeepSee laser (690-1040 nm) in addition to 6 visible spectrum laser lines (405, 458, 488, 514, 561, 633 nm). The multiphoton/two-photon lasers (MP, a category of nonlinear optics, NLO) allow for deeper imaging of fixed tissues or live animals. The Airyscan module offers a superior signal to noise ratio compared to a normal confocal, and combined Fast Airyscan Module delivers high speed comparable to spinning disc confocal. This microscope also includes a special 20× Clarity objective with 5.6 mm working distance specifically for imaging transparent specimens such as CLARITY treated tissues.

In recent years, with the development of optical imaging technology, especially the introduction of digital imaging and computer image analysis, bioimaging has become an indispensable method in cell biology research. CD BioSciences' bioimaging laboratory is committed to providing state-of-the-art technical support to our customers. If you are interested, please do not hesitate to contact us.

For research use only, not intended for any clinical use.

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