Biological Imaging Service—Typhoon FLA 9500 Fluoroimager/Phosphoimager

Biological imaging is an important research tool for understanding tissue structure and elucidating the various physiological functions of living organisms. It uses light or electron microscopy to obtain direct images of the microstructure of biological cells and tissues, and the resulting images are analyzed to understand the various physiological processes of biological cells. CD BioSciences' Bioimaging Centre has dozens of devices and, more importantly, our expertise and experience are invaluable to the industries and clients we serve. We can provide Typhoon FLA 9500 Fluoroimager/Phosphoimager-based bioimaging services.

Name of the Instrument/Platform

Typhoon FLA 9500 Fluoroimager/Phosphoimager

Introduction

Unit Description
1. Excitation wavelengths
1) 473 nm (blue LD laser)
2) 532 nm (green SHG laser)
3) 635 nm (red LD laser)
4) 785 nm (near IR LD laser)
2. Radioisotopes
1) 3H, 11C, 14C, 125I, 18F, 32P, 33P, 35S, ^99mTc and other sources of ionizing radiation
2) Dynamic range-5 orders of magnitude
3) Bit depth-16-bit
4) Scanning area-40 × 46 cm
5) Pixel sizes-10, 25, 50, 100, 200 ?M and prescan 1000 ?M
6) Standard filters- IP (Phosphorimaging), LBP (510LP), LPG (575LP), LPR (665LP), LPR-Ch2 (665LP), BPB1 (530DF20), BPG1 (570DF20) and BPFR800 (R810)
3. Image analysis
1) ImageQuant TL software for 1-D gel electrophoresis, dot blots, arrays, colony counting, and user-defined gel analysis
2) DeCyder 2D Differential Analysis software for high resolution 2-D DIGE analysis including Extended Data Analysis

Feature Highlights

1. Imaging of multifluorescent, chemifluorescent, radioisotope-labeled and calorimetric samples
2. Provides 457/532/635 nm excitation and up to 10 ?m resolution for gel, blot, tissue and array imaging. The system is customized with 785 nm excitation, filters and detectors for multiplex and near infrared fluorescence applications.
3. High resolution and quantitation: a pixel resolution of 10 ?m and a linear signal response over five orders of magnitude provides precise quantitation in gels, blots, tissue sections and arrays
4. High sample throughput: scanning area of 40 × 46 cm enables simultaneous imaging of up to 20 gels or blots measuring 10 × 8 cm in size.
5. 2-D DIGE imaging: simultaneous imaging of two 2-D DIGE gels for differential expression studies
6. Visible and infrared fluorescence imaging: near infrared excitation for imaging IRDye and other infrared dyes.

Summary

Typhoon FLA 9500 is a versatile laser scanner that is ideal for imaging of fluorescent Western Blot membranes. Biomolecular imaging applications include sensitive and quantitative measurements of Western blots, multiplex fluorescence and radioactive labels. It can detect more than one protein at the same time by means of secondary antibodies labeled with different fluorophores. Amersham ECL Plex fluorescence detection systems are compatible with typhoon FLA 9500. It contains the 2-D DIGE system for accurate quantitation of changes in protein expression and the DeCyder 2D differential analysis software. High sensitivity and broad dynamic range make it highly suitable for 2-D DIGE applications to detect and quantitate subtle changes in protein expression.

In recent years, with the development of optical imaging technology, especially the introduction of digital imaging and computer image analysis, bioimaging has become an indispensable method in cell biology research. CD BioSciences' bioimaging laboratory is committed to providing state-of-the-art technical support to our customers. If you are interested, please do not hesitate to contact us.

For research use only, not intended for any clinical use.

Related Services