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Bioimaging Service—Zeiss Sigma 300 FEG-SEM

Transmission electron microscopy is an important tool for the observation and study of ultrastructure and is widely used in a variety of disciplines such as histology, cytology, virology, pathology, and materials science, for example, to observe the overall structure of cells, changes in cell membranes, organelles in cell walls, the distribution of materials into cells, autophagic vesicles produced by cells in response to external stimuli, and invasive structures invaded by external organisms. CD BioSciences' Zeiss Sigma 300 FEG-SEM-based bioimaging service enables customers to see the microscopic world.

Name of the Instrument/Platform

Zeiss Sigma 300 FEG-SEM

Feature Highlights

Accelerating voltage: 0.02 kV to 30 kV
Resolution: 1.2 nm (at 15 kV), 2.2 nm (at 1 kV)
Magnification 10x to 1,000,000x
Schottky Thermal Field Emitter electron source
Everhart-Thornley and Inlens secondary electron detectors
High Definition Backscattered detector
STEM detector for imaging ultrathin samples on TEM grids
Oxford Instruments Nanoanalysis Ultim® Extreme EDS detector for detection and mapping of low energy characteristic x-rays
Chamber scope CCD camera
5-axis motorised Cartesian stage
Frame size: up to 3072 x 2304 pixels

Applications

Detailed characterisation of mutant phentoypes of whole organisms (eg: HeLa cells, Drosphilia, C. elegans and Arabidopsis)
Examination of particulate samples (eg: pollen, powder, magnetic microparticles) and polymers
Characterisation of the effect of drugs on mammalian culture cells etc
Observation of cells grown on artificial material (eg: scaffolds)

Summary

The Zeiss Sigma 300 Field Emission Gun Scanning Electron Microscope (FEG-SEM) enables high resolution imaging of biological and biomaterial samples, providing surface structural, topographical information, in addition to compositional contrast. The Sigma 300 offers a range of features for easy acquisition of high quality SEM micrographs.

CD BioSciences offers you Zeiss Sigma 300 FEG-SEM-based bioimaging services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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