Bioimaging Service—TIRF 3 Nikon-Roper ILAS-2 PhotoAblation

CD BioSciences is committed to providing one-stop services. Bioimaging service fully meets the needs of our clients to help them understand the tissue structure and elucidate the various physiological functions of living organisms. We can offer a wide range of bioimaging services based on TIRF 3 Nikon-Roper ILAS-2 PhotoAblation.

Name of the Instrument/Platform

TIRF 3 Nikon-Roper ILAS-2 PhotoAblation

Introduction

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)
Microscope: Nikon Eclipse Ti with Perfect Focus System

Feature Highlights

Objective: Nikon Apo TIRF 100x N.A. 1.49 oil
Beamsplitter: Optosplit III (allows two or three color simultaneous imaging)
Camera:
Photometrics Evolve Delta 512 EMCCD
Photometrics CoolSNAP Myo CCD
Lasers:
Vortran Stradus 405 (405 nm / 100 mW)
Vortran Stradus 445 (445 nm / 80 mW)
Coherent OBIS 561-100LS (561 nm / 100 mW)
Vortran Stradus 488 (488 nm / 150 mW)
Vortran Stradus 642 (642 nm / 110 mW)
Teem Photonics 532 nm (for photoablation)
FRAP/TIRF: Ilas-2 system (Roper Scientific France/ PICT-IBiSA, Institut Curie)
Filters:
ET-BFP2 (49021), ET-GFP (49002), ET-mCherry (49008), ET-GFPmCherry (59022) – all Chroma
Stage: ASI motorized stage MS-2000-XY System for Inverted Microscope Nikon Te/Ti 2000
Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; emission filter wheel; smart shutter for transmission light)
Heating: Tokai Hit INUBG2E-ZILCS Stage Top Incubator
Software: MetaMorph 7.8
Resolution: 65 nm in 1 pixel

CD BioSciences offers you pharmaceutical analysis services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

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