Bioimaging Service—TIRF 2 Nikon-Roper ILAS PhotoAblation

CD BioSciences is committed to providing one-stop services. Bioimaging service fully meets the needs of our clients to help them understand the tissue structure and elucidate the various physiological functions of living organisms. We can offer a wide range of bioimaging services based on TIRF 2 Nikon-Roper ILAS PhotoAblation.

Name of the Instrument/Platform

TIRF 2 Nikon-Roper ILAS PhotoAblation

Introduction

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)
Microscope: Nikon Eclipse Ti with Perfect Focus System

Feature Highlights

Objective: Nikon Apo TIRF 100x N.A. 1.49 oil
Beamsplitter: Optosplit III (allows two or three color simultaneous imaging)
Camera:
Photometrics Evolve 512 EMCCD
Photometrics CoolSNAP HQ2 CCD
Lasers:
Vortran Stradus 405 nm (100 mW)
488 nm (150 mW)
Cobolt Jive 561 nm (100 mW)
Vortran Stradus 642 nm (110 mW)
Teem Photonics 532 nm Q-switched pulsed laser (for photoablation)
FRAP: ILas system (Roper Scientific France/ PICT-IBiSA, Institut Curie)
Filters:
ET-DAPI (49000), ET-CFP (49001), ET-GFP (49002), ET-YFP (49003), ET-DSRed (49005), ET-Cy5 (49006), ET-mCherry (49008), ET-CFP/YFP (59017), ET-GFPmCherry (59022) – all Chroma
Stage: ASI motorized stage MS-2000-XY System for Inverted Microscope Nikon Te/Ti 2000
Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; emission filter wheel; smart shutter for transmission light)
Heating: Tokai Hit INUBG2E-ZILCS Stage Top Incubator
Software: MetaMorph 7.7
Resolution: 63 nm in 1 pixel

CD BioSciences offers you pharmaceutical analysis services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

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