Bioimaging Service—TIRF 1 Nikon-Roper ILAS

CD BioSciences is committed to providing one-stop services. Bioimaging service fully meets the needs of our clients to help them understand the tissue structure and elucidate the various physiological functions of living organisms. We can offer a wide range of bioimaging services based on TIRF 1 Nikon-Roper ILAS.

Name of the Instrument/Platform

TIRF 1 Nikon-Roper ILAS

Introduction

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Multi-position acquisition,
Electrical Stimulation, Perfusion Experiments, Phase Contrast Time Lapse Microscopy

Microscope: Nikon Eclipse TE2000 with Perfect Focus System

Feature Highlights

Objectives:
Apo TIRF 100x/1.49 oil, Plan Apo VC 100x/1.4 oil, Plan Fluor 40x/1.30 oil, Plan Apo 40x/1.0 oil,
Plan Apo 20x/0.75 dry
Beamsplitter: DV 2 (two-channel, simultaneous-imaging system)
Camera:
Photometrics Evolve 512 EMCCD
Photometrics CoolSNAP HQ2 CCD
Lasers:
Vortran 405 nm (100 mW)
Cobolt Calypso 491 nm (100 mW)
Cobolt Jive 561 nm (100 mW)
FRAP: ILas0 system (Roper Scientific France/Institut Curie)
Filters:
ET-DAPI (49000), ET-CFP (49001), ET-GFP (49002), ET-YFP (49003), ET-DSRed (49005), ETCy5
(49006), ET-mCherry (49008), ET-CFP/YFP (59017), ET-GFPmCherry (59022) – all Chroma
Stage: Prior
Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; emission filter wheel; smart shutter for transmission light)
Heating/CO2: Tokai Hit INUG2E-ZILCS Stage Top Incubator
Software: MetaMorph 7.7

CD BioSciences offers you pharmaceutical analysis services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

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