Bioimaging Service—Spinning Disc 1 Nikon-Roper ILAS PhotoAblation

CD BioSciences is committed to providing one-stop services. Bioimaging service fully meets the needs of our clients to help them understand the tissue structure and elucidate the various physiological functions of living organisms. We can offer a wide range of bioimaging services based on Spinning Disc 1 Nikon-Roper ILAS PhotoAblation.

Name of the Instrument/Platform

Spinning Disc 1 Nikon-Roper ILAS PhotoAblation

Introduction

Primary tasks:
Confocal Spinning Disc microscopy, 3D imaging, FRAP, Photo-Activation, Photo-Ablation (355 nm)
Microscope: Nikon Eclipse Ti with Perfect Focus System
Confocal Head: Yokogawa CSU-X1-A1

Feature Highlights

Objective:
Apo TIRF 100x N.A. 1.49 oil, S Fluor 100x N.A. 0.5-1.3 (for 355 nm) oil, Plan Apo VC 100x N.A. 1.40 oil, Plan Apo VC 60x N.A. 1.40 oil, Plan Fluor 40x N.A. 1.30 oil, Plan Apo 20x N.A. 0.75, Plan Apo 10x N.A. 0.45

Beamsplitter: DV2 (Two-channel, simultaneous-imaging system)

Camera: Photometrics Evolve 512 EMCCD
Lasers:
Vortran Stradus 405 (405 nm / 100 mW)
Vortran Stradus 445 (445 nm / 80 mW)
Vortran Stradus 488 (448 nm / 150 mW)
Cobolt Jive (561 nm / 110 mW)
Vortran Stradus 642 (642 nm / 165 mW)
Teem Photonics 355 nm Q-switched pulsed laser (for photoablation)
FRAP: ILas system (Roper Scientific France/ PICT-IBiSA, Institut Curie)
Filters:
ET-DAPI (49000), ET-CFP (49001), ET-GFP (49002), ET-YFP (49003), ET-mCherry (49008), ET-CFP/YFP (59017), ET-GFPmCherry (59022) – all Chroma
Stage: ASI motorized stage MS-2000-XYZ with Piezo Top Plate
Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; smart shutter for transmission light)
Heating: Tokai Hit INUBG2E-ZILCS Stage Top Incubator
Software: MetaMorph 7.7
Resolution: 67 nm in 1 pixel (100x objective)

CD BioSciences offers you pharmaceutical analysis services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

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