Bioimaging Service—Serial Block Face Sectioning SEM

Transmission electron microscopy is an important tool for the observation and study of ultrastructure and is widely used in a variety of disciplines such as histology, cytology, virology, pathology, and materials science, for example, to observe the overall structure of cells, changes in cell membranes, organelles in cell walls, the distribution of materials into cells, autophagic vesicles produced by cells in response to external stimuli, and invasive structures invaded by external organisms. CD BioSciences' Serial Block Face Sectioning SEM-based bioimaging service enables customers to see the microscopic world.

Name of the Instrument/Platform

Serial Block Face Sectioning SEM

Feature Highlights

1 nm resolution at 15 kV
Variable pressure mode (10-60 Pa) to mitigate charging on beam reactive samples
In-built plasma cleaner
Gatan 3View 2 XP system with diamond knife for 15 nm to 200 nm slices and up to 600 µm volume
Up to 32k x 32k frame size

Applications

3D ultrastructural imaging of large volumes at high resolution (eg: model the entire mitochondrial network within a cell or trace a dendrite through hundreds of microns of brain matter)
Correlative 3D light and electron microscopy using the 3View or array tomography of serial sections

Summary

This cutting-edge microscope contains an in situ ultramicrotome which enables the collection of large, high resolution volumes of resin embedded biological samples using a technique called serial block face sectioning.

CD BioSciences offers you Serial Block Face Sectioning SEM-based bioimaging services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.

Related Services