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Bioimaging Service—Olympus Fluoview FV1200

Transmission electron microscopy is an important tool for the observation and study of ultrastructure and is widely used in a variety of disciplines such as histology, cytology, virology, pathology, and materials science, for example, to observe the overall structure of cells, changes in cell membranes, organelles in cell walls, the distribution of materials into cells, autophagic vesicles produced by cells in response to external stimuli, and invasive structures invaded by external organisms. CD BioSciences' Olympus Fluoview FV1200-based bioimaging service enables customers to see the microscopic world.

Name of the Instrument/Platform

Olympus Fluoview FV1200

Feature Highlights

FluoView Spectral FV1200 Laser Scanning Microscope based on a IX83 motorized inverted microscope
CellVivo incubator system for live-cell imaging and temperature control
6 laser lines
3 standard PMT and 2 high sensitive GaAsP detectors.
Freely selectable ROI for laser scanning, bleaching and photoactivation
Scan speeds of up to 4 frames/second with 512 x 512 pixels
xy, xz, xyz, xyzt, lambda, line scan and spot scan modes

Applications

Imaging fixed tissue culture cells tissue sections labelled with 3 or 4 fluorophores for cellular architecture, localisation or co-localisation studies.
Imaging of thicker samples such as organoids, embryos etc.
‘SIM' scan-head for simultaneous imaging and laser ablation (DNA damaging).
Environmental chamber for time-lapse imaging at 37oC with 5% CO2.
Programmable stage for imaging larger structures such as organ slices, or multiple points on a slide.

Summary

The FV1200 confocal is an inverted IX83 microscope equipped with sensitive GASP detectors, a second 'SIM' scan-head for DNA damage laser experiments and an environmental chamber for live cell work. The motorized stage can be used for image stitching of large samples and time-lapse imaging at multiple positions. Accompanying Imaris Software is available for 3D image reconstruction.

CD BioSciences offers you Olympus Fluoview FV1200-based bioimaging services to help you solve your research problems related to proteomics, metabolomics, lipidomics, and more. If you are interested, please contact us directly.

For research use only, not intended for any clinical use.
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CD BioSciences is committed to providing a tailored service to researchers working in the fields of biology and chemistry. Our mission is to accelerate the progress of your research through our technical support.

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