Animal Imaging Service—IVIS^® Spectrum (Perkin Elmer)

CD BioSciences' animal imaging technology platform has multiple state-of-the-art instruments to study cardiovascular function, skeletal morphology, metabolism, and more by performing ultrasound and computed tomography imaging on live animals. Our IVIS^® Spectrum (Perkin Elmer)-based animal imaging service is the right choice for you.

Name of the Instrument/Platform

IVIS^® Spectrum (Perkin Elmer)

MANUFACTURER:

Perkin

Introduction

Equipment specifications
1. CCD camera
1) Sensor type: Back illuminated
2) Format: 2048 × 2048 pixels
3) Size: 26 × 26cm
4) Effective Format: 1920 × 1920 pixels
5) Pixel Dimensions: 13.5 × 13.5 μm
6) Quantum Efficiency: ~85% 400-700nm and >50% 350-900nm
7) Readout Noise: < 5 electrons RMS
8) Dark Current: < 100 electrons/s/cm² (-90℃)
9) Minimum Detectable Luminance: < 70 photons/s/sr/cm²
10) Temperature: Normal -90℃
2. Optics
1) Lens f/stop: f/1 - f/8
2) Field of View (FOV): 3.9, 6.5, 13, 19.5, and 26 cm
3) Resolution: >60 μm (FOV = 3.9, f/1)
3. Fluorescence Imaging Components
1) Excitation Filters: 12 positions, 25 mm diameter, 10 filters supplied
2) Emission Filters: 22 positions, 60 mm diameter, 18 filters supplied
3) Wavelengths spanning from 430 nm to 850 nm
4) Background: 1 part in 107 typical

Feature Highlights

1. The machine is very easy to use, usually requiring only 30 minutes of training.
2. IVIS Spectrum is a flexible and highly sensitive instrument for both bioluminescent and fluorescent imaging.
3. Wide range of reporters and fluorophores available for imaging (cellular and molecular level)
4. Possibility of imaging multiple targets together (spectral unmixing)
5. Imaging up to 5 mice at once
6. High throughput capability
7. Longitudinal studies

Applications

1. Biomedical research including oncology, infectious disease, musculoskeletal, cardiovascular, neurology, respiratory and stem cell research.
2. Pharmacokinetic/pharmacodynamic studies and analysis
3. Cancer imaging- tumor size (growth) and markers
4. Cell trafficking
5. Gene expression
6. Drug development
7. Detection of enzymatic activity and cell viability
8. Biodistribution and proliferation of organisms in infection models

Summary

The IVIS Spectrum is equipped with filters enabling both bioluminescence and fluorescence 2D and 3D imaging. The equipment is operated through Living Image software which allows you to choose from 3 basic types of imaging: imaging of 1 target, spectral unmixing for imaging multiple targets, and 3D imaging. Using automatic settings allows for easy and comparable imaging that can be quantified both in 2D and 3D. Choice of different ROI types are available for in vivo, ex vivo, and in vitro imaging. The equipment gives the option of imaging up to 5 mice per image.

If you need to perform imaging studies on animals such as mice, please contact us directly for details.

For research use only, not intended for any clinical use.

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Animal Imaging Service—IVIS® Spectrum (Perkin Elmer)